A new, accurate, precise, and rapid RP-HPLC method has been developed and validated for the simultaneous estimation of diacerein and celecoxib in capsule dosage. The separation was achieved by using C18 column (Thermo scientific, 250×4.6 mm, 5 μm Particle Size) and the mobile phase consisting of 0.02 M potassium dihydrogen phosphate (containing 0.1% triethylamine and pH adjusted to 3.50±0.05 using 20% orthophosphoric acid) and acetonitrile and in the proportion of 60:40 (v/v/v). Detection was carried out at 255 nm. Retention time of celecoxib and diacerein was found to be 5.72±0.67 and 3.76±0.5 min, respectively. The method has been validated for linearity, accuracy and precision and robustness. Linearity for celecoxib was in the range of 20-60 μg/ml and for diacerein in the range of 5-15 μg/ml. The percentage recoveries obtained for celecoxib and diacerein were in range of 99.54-100.65% and 98-100.41% respectively and can be successfully applied for the routine analysis in pharmaceutical capsule dosage form.
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